Gunma University Initiative for Advanced Research > Laboratory > Endocrinology, Metabolism and Signal Research > Dr. SHIBATA Laboratory

Dr. SHIBATA Laboratory

DNA Repair Studies

How can damaged DNA be repaired?
Unravel the mysteries of DNA repair, the force to accurately restore DNA!

Research Fields

Molecular biology, cancer medicine

Keywords

DNA repair, fluorescence imaging analysis, cancer therapy, cancer immunotherapy

Why is DNA repair research necessary?

Actually, the DNA of the human body is damaged at a high rate every day. However, the human body is able to conduct DNA repair, namely to accurately restore damaged DNA. Our laboratory regards DNA repair as a single discipline, and our aim is to contribute to the promotion of human health by eludicating DNA repair mechanisms and applying them to various medical treatments.

We need to know more about this reaction system because whether or not DNA (the blueprint of the human body) can be properly repaired, obviously affects the entire human body. We are trying to elucidate how cells select the best DNA repair pathway and maintain a stable genome. In particular, we are trying to visualize DNA repair in living cells, which was impossible with previous techniques, by taking advantage of new technologies, including super-resolution microscopy.

We are conducting research on how DNA repair reactions that occur during cancer treatment affect the effectiveness of treatment. For example, radiotherapy and chemotherapy, which are performed during cancer treatment, kill cancer cells by damaging their DNA. In other words, we believe that we can contribute to cancer treatment by clarifying how DNA damage that occurs during cancer treatment is repaired or not repaired.

We pursue research every day, believing that the elucidation of mechanisms that are related to DNA repair, which is fundamental for the maintenance of life, will lead to the development of various medical treatments, including cancer therapy.

Research Policy of the Shibata Lab

Papers

  • Takaaki Yasuhara#*, Reona Kato#, Motohiro Yamauchi, Yuki Uchihara, Lee Zou, Kiyoshi Miyagawa*, Atsushi Shibata*
    RAP80 suppresses the vulnerability of R-loops during DNA double-strand break repair
    Cell Reports, 38, 110335, 2022
    *Co-corresponding authors, #Co-first
  • Takaaki Yasuhara#*, Reona Kato#, Yoshihiko Hagiwara, Bunsyo Shiotani, Motohiro Yamauchi, Shinichiro Nakada, Atsushi Shibata* and Kiyoshi Miyagawa*.
    Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair.
    Cell, 175 (2):558-570, 2018.
    *Co-corresponding author
  • Hiro Sato, Atsuko Niimi, Takaaki Yasuhara, Tiara Bunga Mayang Permata, Yoshihiko Hagiwara, Mayu Isono, Endang Nuryadi, Ryota Sekine, Takahiro Oike, Sangeeta Kakoti, Yuya Yoshimoto, Kathryn D. Held, Yoshiyuki Suzuki, Koji Kono, Kiyoshi Miyagawa, Takashi Nakano and Atsushi Shibata*.
    DNA double-strand break repair pathway regulates PD-L1 expression in cancer cells
    Nature Communications, 8(1):1751, 2017.
    *Corresponding author
  • Mayu Isono, Atsuko Niimi, Takahiro Oike, Yoshihiko Hagiwara, Hiro Sato, Ryota Sekine, Yukari Yoshida, Shin-Ya Isobe, Chikashi Obuse, Ryotaro Nishi, Elena Petricci, Shinichiro Nakada, Takashi Nakano and Atsushi Shibata*. BRCA1 directs the repair pathway to homologous recombination by promoting 53BP1 dephosphorylation. Cell Reports, 18(2):520-532, 2017.
    *Corresponding author

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